<object id="q8oyw"></object>
<sup id="q8oyw"><option id="q8oyw"></option></sup><object id="q8oyw"><tr id="q8oyw"></tr></object>
<center id="q8oyw"><center id="q8oyw"></center></center>
<object id="q8oyw"></object>
<acronym id="q8oyw"><div id="q8oyw"></div></acronym>
<object id="q8oyw"></object>
<acronym id="q8oyw"><wbr id="q8oyw"></wbr></acronym>
<object id="q8oyw"></object>
咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CCL-138Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

簡(jiǎn)要描述:Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CCL-138
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:1437

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

OrganismHomo sapiens, human
Tissuepharynx: derived from metastatic site: pleural effusion
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level1
Diseasepharyngeal carcinoma
Age*****
Genderfemale
EthnicityCaucasian
Storage Conditionsliquid nitrogen vapor phase

 Karyotype   Modal number = 64; range = 58 to128
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines   Clinical Data  

Detroit 562細(xì)胞, 人咽頭癌胸水轉(zhuǎn)移細(xì)胞

Caucasian

female

 Genes Expressed  

keratin

 Virus Susceptibility   Human poliovirus 1
Vesicular stomatitis virus              
 Comments  

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing





Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage Temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: Air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C              CCL-138 Detroit 562 人咽頭癌胸水轉(zhuǎn)移細(xì)胞























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
<object id="q8oyw"></object>
<sup id="q8oyw"><option id="q8oyw"></option></sup><object id="q8oyw"><tr id="q8oyw"></tr></object>
<center id="q8oyw"><center id="q8oyw"></center></center>
<object id="q8oyw"></object>
<acronym id="q8oyw"><div id="q8oyw"></div></acronym>
<object id="q8oyw"></object>
<acronym id="q8oyw"><wbr id="q8oyw"></wbr></acronym>
<object id="q8oyw"></object>
铜山县| 鄂温| 涟水县| 西和县| 金湖县| 武川县| 静宁县| 涪陵区| 盈江县| 茂名市| 西乌| 石狮市| 遵化市| 隆德县| 罗城| 远安县| 罗城| 阳原县| 前郭尔| 临颍县| 昂仁县| 湘潭市| 浦东新区| 九台市| 凌云县| 安乡县| 双牌县| 德江县| 甘洛县| 永泰县| 栾城县| 扶沟县| 晋宁县| 曲麻莱县| 漳州市| 莱西市| 景谷| 河津市| 运城市| 永靖县| 剑阁县| http://444 http://444 http://444 http://444 http://444 http://444