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Calu-3 人類肺腺癌細(xì)胞(胸水)

簡(jiǎn)要描述:Calu-3 人類肺腺癌細(xì)胞(胸水)
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Calu-3 人類肺腺癌細(xì)胞(胸水)

ATCC® Number:HTB-55™    

Designations:Calu-3

Depositors:J Fogh

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: lung
Disease: adenocarcinoma
Derived from metastatic site: pleural effusion


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Calu-3 人類肺腺癌細(xì)胞(胸水)

Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Tumorigenic:Yes

Antigen Expression:Blood Type A; Rh+

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 12

D16S539: 12,14

D5S818: 11

D7S820: 10,11

THO1: 6,9.3

TPOX: 8

vWA: 16,17



Cytogenetic Analysis:The stemline chromosome number is hypotriploid with the 2S component occurring at 1.4%. Approximay 20 markers were common to most S metaphases, of which i (1p), t(12;?) and t(18;?) were generally paired; t(6t?) had an HSR segment of q arm, and M7 had two secondary constrictions. Normal chromosomes 1, 13, 15 and 17 were absent, and the X was disomic. No Y chromosome was detected in the QM stained preparations.

Isoenzymes:AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1-2



Age:25 years

Gender:male

Ethnicity:Caucasian

Comments:The patient had received prior therapy with cytoxan, bleomycin and adriamycin.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week




MLE-12 小鼠肺上皮細(xì)胞

saos-2 人成骨肉瘤細(xì)胞

MA104 非洲綠猴胚胎腎上皮細(xì)胞

ball-1 細(xì)胞

nci-h226 人肺鱗癌細(xì)胞

MCA38 小鼠結(jié)腸癌細(xì)胞

LTPA 小鼠胰腺癌細(xì)胞

NCI-H23 人非小細(xì)胞肺癌細(xì)胞

293.00     人胚腎細(xì)胞

293FT    人胚腎細(xì)胞-用于慢病毒包裝

293TN    人胚腎細(xì)胞--用于慢病毒包裝

293AV    人胚腎細(xì)胞-用于腺病毒包裝

293T    人胚腎細(xì)胞

208F    大鼠成纖維細(xì)胞

786-O    人腎透明細(xì)胞腺癌細(xì)胞

A172    人腦膠質(zhì)母細(xì)胞瘤細(xì)胞

A549    人肺癌細(xì)胞 細(xì)胞系購(gòu)買

AGS    人胃癌細(xì)胞

BEL7402 人肝癌細(xì)胞系

AZ-521    人胃癌細(xì)胞

B16F0    小鼠黑色素瘤細(xì)胞

Bel-7402    人肝癌細(xì)胞

Bel-7404    人肝癌細(xì)胞

BGC-823    人胃癌細(xì)胞

C6    大鼠腦膠質(zhì)瘤細(xì)胞

SW480    人結(jié)腸腺癌細(xì)胞

CaCo-2    人結(jié)腸癌細(xì)胞

Cho-K1    倉(cāng)鼠卵巢細(xì)胞亞株

COS-7    非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞

GES-1    人胃上皮細(xì)胞

HCT-8    人回盲腸癌細(xì)胞

HeLa

Hepa1-6    小鼠肝癌細(xì)胞

HepG2    人肝癌細(xì)胞

hFOB1.19    人永生化成骨細(xì)胞

HGC-27    人胃癌細(xì)胞

Hs 746T    人胃癌細(xì)胞

HT-29    人結(jié)腸癌細(xì)胞

KB    人口腔表皮樣癌細(xì)胞

LoVo    人結(jié)腸癌細(xì)胞

LLC-PK1    豬腎近曲小管上皮細(xì)胞

MCF-10A    人正常乳腺上皮細(xì)胞

MCF-7    人乳腺癌細(xì)胞

MDA-MB-231    人乳腺癌細(xì)胞

MGC80-3    人胃癌細(xì)胞

MKN-45    人胃癌細(xì)胞

NCI-H460    人大細(xì)胞肺癌細(xì)胞

NIH-3T3    小鼠成纖維細(xì)胞

NUGC-3    人胃癌細(xì)胞

Raw264-7    小鼠單核細(xì)胞白血病細(xì)胞

SGC-7901    人胃癌細(xì)胞

SMMC-7721    人肝癌細(xì)胞

SNU-638    人胃癌細(xì)胞

SNU-739    人肝癌細(xì)胞

Tca8113    人舌鱗癌細(xì)胞    細(xì)胞系購(gòu)買

U251    人腦膠質(zhì)瘤細(xì)胞

U-373MG    人腦神經(jīng)膠質(zhì)瘤細(xì)胞

U-87 MG    人腦星形膠質(zhì)母細(xì)胞瘤

BT-474    人乳腺導(dǎo)管癌細(xì)胞

PANC-1    人胰腺癌細(xì)胞

HEK293    人胚腎細(xì)胞

Hep3B    人肝癌細(xì)胞

HL-7702    人正常肝細(xì)胞

Raw-Blue    小鼠單核細(xì)胞白血病細(xì)胞

WEHI-231    小鼠B淋巴細(xì)胞

OPM-2    人骨髓瘤細(xì)胞

NCI-H929    人骨髓瘤細(xì)胞

U266    人骨髓瘤細(xì)胞

EJ    人膀胱癌細(xì)胞

HOC    大鼠肝卵圓細(xì)胞

RWPE-1    人正常前列腺上皮細(xì)胞

CAL 27    人舌鱗癌細(xì)胞

Calu-3    人肺腺癌細(xì)胞

Eca-109    人食管癌細(xì)胞

DLD-1    人結(jié)直腸腺癌上皮細(xì)胞

5637.00     人膀胱癌細(xì)胞

SK-OV-3    人卵巢癌細(xì)胞

GBC-SD    人膽囊癌細(xì)胞

BEL7402 人肝癌細(xì)胞系

SW620    人結(jié)腸癌細(xì)胞

MRC-5    人胚肺成纖維細(xì)胞     細(xì)胞系購(gòu)買

PC9    人肺癌細(xì)胞

PC14    人肺癌細(xì)胞

BxPC    人原位胰腺腺癌細(xì)胞

PK(15)    豬腎上皮細(xì)胞

MDCK    狗腎上皮細(xì)胞

3D4/21    豬肺泡巨噬細(xì)胞

MHCC-97L    人肝癌細(xì)胞(低轉(zhuǎn)移)

MHCC-97H    人肝癌細(xì)胞系(高轉(zhuǎn)移)

MiaCaPa-2    人胰腺癌細(xì)胞

HN13    口腔鱗狀細(xì)胞癌細(xì)胞

Bend3    小鼠腦微血管內(nèi)皮細(xì)胞

QBC939    人膽管癌細(xì)胞

Ishikawa    人子宮內(nèi)膜癌細(xì)胞

A498    人腎癌細(xì)胞

143B

WEHI-231    小鼠B淋巴細(xì)胞

H322    人非小細(xì)胞肺癌細(xì)胞

Panc O2

S8    BEL7402 人肝癌細(xì)胞系

R5    細(xì)胞系購(gòu)買

Pig MSC    豬骨髓間質(zhì)干細(xì)胞

HCAEC    人冠狀動(dòng)脈內(nèi)皮細(xì)胞

marc-145    猴胚胎腎上皮細(xì)胞

3D4/21    豬肺泡巨噬細(xì)胞

PK(15)    豬腎上皮細(xì)胞

MADB-106    大鼠乳腺癌細(xì)胞

Sf9    昆蟲(chóng)卵巢細(xì)胞

DADY

HUVEC[HUV-EC-C]    人臍靜脈內(nèi)皮細(xì)胞

PC14    人肺癌細(xì)胞

PC9    人肺癌細(xì)胞

Eca-109    人食管癌細(xì)胞

CAL 27    人舌鱗癌細(xì)胞

HOC    肝卵圓細(xì)胞

EJ    人膀胱癌細(xì)胞

U266    人骨髓瘤細(xì)胞

293-mTLR5    人胚腎細(xì)胞

Raw-Blue    小鼠單核細(xì)胞白血病細(xì)胞

HL-7702    人正常肝細(xì)胞

Hep3B    人肝癌細(xì)胞

U-373MG    人腦神經(jīng)膠質(zhì)瘤細(xì)胞

Tca-8113    人舌鱗癌細(xì)胞

SNU-739    人肝癌細(xì)胞

SNU-638    人胃癌細(xì)胞

NUGC-3    人胃癌細(xì)胞

NIH-3T3    小鼠成纖維細(xì)胞

MKN-45    人胃癌細(xì)胞

LLC-PK1    豬腎近曲小管上皮細(xì)胞

Hs 746T    人胃癌細(xì)胞

Hepa1-6    小鼠肝癌細(xì)胞

HCT-8    人回盲腸癌細(xì)胞

BEL7402 人肝癌細(xì)胞系

HaCaT    人永生化表皮細(xì)胞

GES-1    人胃上皮細(xì)胞

B16-F0    小鼠黑色素瘤細(xì)胞

AZ-521    人胃癌細(xì)胞

786-O    人腎透明細(xì)胞腺癌細(xì)胞

208F    大鼠成纖維細(xì)胞

293TN    人胚腎細(xì)胞--用于慢病毒包裝

293FT    人胚腎細(xì)胞-用于慢病毒包裝

GP-293    人胚腎細(xì)胞

293-L.P.    人胚腎細(xì)胞

HTB-55 Calu-3 人類肺腺癌 (胸水)
















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